LeHavreV1, Main, Exploration, bibRecord, 000D55

Detection of environmental mutagens using the FACIM assay.

Identifieur interne : 000D55 ( Main/Exploration ); précédent : 000D54; suivant : 000D56

Detection of environmental mutagens using the FACIM assay.

Auteurs : J. Couteau [France] ; J-M Flaman ; C. Minier ; J. Cachot

Source :

Marine environmental research [ 0141-1136 ] ; 2008.

RBID : pubmed:18420266

English descriptors

KwdEn :
- Environmental Monitoring (methods), Geologic Sediments (chemistry), Mutagenicity Tests (methods), Mutation (drug effects), Regression Analysis, Yeasts (drug effects), Yeasts (genetics).
MESH :
- chemistry : Geologic Sediments.
- drug effects : Mutation, Yeasts.
- genetics : Yeasts.
- methods : Environmental Monitoring, Mutagenicity Tests.
- Regression Analysis.

Abstract

A genetically engineered diploid yeast strain named yJC2, was specifically developed for environmental mutagen detection and characterization of induced mutations. This strain contains one copy of the human TP53 tumour suppressor gene coding sequence which is used as a molecular target for mutagens and two copies of the ADE2 reporter gene allowing accurate measurement of the TP53 transcriptional activity. The strain sensitivity to mutagens was evaluated by exposing cells to UVC, 4-nitroquinoline (NQO) or to an organic extract of sediment from the Seine estuary. For all studied mutagens, a significant and dose-dependent increase of mutant frequency was observed. The present assay named FACIM II (Functional Analysis of Chemical-Induced TP53 Mutations) is more convenient than the FACIM I and more inducible than the SOS Chromotest to detect direct-acting mutagens in the environment.

DOI: 10.1016/j.marenvres.2008.02.023
PubMed: 18420266

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">A genetically engineered diploid yeast strain named yJC2, was specifically developed for environmental mutagen detection and characterization of induced mutations. This strain contains one copy of the human TP53 tumour suppressor gene coding sequence which is used as a molecular target for mutagens and two copies of the ADE2 reporter gene allowing accurate measurement of the TP53 transcriptional activity. The strain sensitivity to mutagens was evaluated by exposing cells to UVC, 4-nitroquinoline (NQO) or to an organic extract of sediment from the Seine estuary. For all studied mutagens, a significant and dose-dependent increase of mutant frequency was observed. The present assay named FACIM II (Functional Analysis of Chemical-Induced TP53 Mutations) is more convenient than the FACIM I and more inducible than the SOS Chromotest to detect direct-acting mutagens in the environment.</div>
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Detection of environmental mutagens using the FACIM assay.

Detection of environmental mutagens using the FACIM assay.

Source :

English descriptors

Abstract

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Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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